anti-ck18/fitc
catalog number : rsf-1059r-fitc
quantity size : 100ul (2mg/ml,lyophilized. buffer = 0.01m pbs, ph 7.4 with 10mg/ml bsa and 0.1% sodium azide. )
background: cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. cytokeratin 18 exists in combination with cytokeratin 8, a basic keratin. hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only cytokeratins 8 and 18.
specificity : · anti- ck18 is a mouse monoclonal antibody, /fitc
· specific for ck18 of human
· protein g affinity chromatography purification, purity :>95%
· isotype: igg
· mol wt:48kda
application : .if=1:50-200
· immunohistochemistry : 1:20-1:200
· immunocytochemistry: 1:20-1:200
· excitation spectrum: 490-495nm
· emission spectrum: 520-530nm
. klh conjugated synthetic peptide derived from human ck18 (53-96aa)
· optimal working dilutions must be determined by the end user.
· protect from light.
storage: store at –20 ºc for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºc. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºc.
important note: this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.