线粒体膜电位（mitochondrial membrane potential,mmp）是判定细胞健康程度、线粒体膜通透性和细胞凋亡的一个重要指标，mmp的丧失通常与细胞凋亡的早期阶段有关。评估线粒体功能状态的基于细胞的检测方法正在成为阐明线粒体活动在药物诱导毒性、细胞凋亡级联以及其他细胞和生化过程中的作用的有用工具。
enzo life sciences的mito-id®membrane potential detection kit包含一种双发射阳离子染料，用于检测活细胞中的线粒体膜电位（mmp）。在有能量或活跃的细胞中，mito-id®膜电位试剂因其相对负电荷而在线粒体中迅速聚集成发橙色荧光的聚合体，而在细胞质中则以发绿色荧光的单体存在。然而，在mmp受损的细胞中，mito-id®膜电位试剂主要以绿色荧光单体存在于整个细胞质中，在线粒体中不再表现出橙色荧光。
作用机制
该染料的基本化学结构由高度共轭的部分组成，使正电荷广泛离域。该染料能够选择性地进入线粒体，当膜电位增加时，它的颜色会从绿色可逆地变为橙色（双发射电位探针）。这种光物理特性是由于在膜极化时可逆地形成j-聚集体，导致在490nm处激发时，发射光从～530nm转移到590nm。因此，具有低膜电位的线粒体将积累低浓度的染料，并表现出绿色荧光，而更高极化的线粒体将表现出橙色的荧光。
mito-id®membrane potential detection kit线粒体膜电位检测试剂盒
产品特点
●耐光双发射染料，能够根据线粒体膜电位状态发出绿色或橙色荧光
●比jc-1荧光染料灵敏10倍，并具有卓-越的水溶性
●无需洗涤步骤
●适用于化学/环境毒性筛选
●适用于高通量应用
如需购买enzo产品，或咨询产品技术问题，请联系enzo life science代理商欣博盛生物
实验示例
图1.用mito-id®membrane potential reagent对hela细胞的线粒体进行染色，并通过荧光显微镜进行观察。橙色荧光聚集体定位于线粒体（橙色通道），而绿色荧光单体主要定位于细胞质（fitc通道）。
图2.对照组和实验组细胞的流式细胞仪分析。未经处理的jurkat细胞（左）与用1μm cccp处理15分钟的juekat细胞（右），用mito-id®membrane potential reagent对细胞进行染色，并使用流式细胞仪检测。
产品信息
产品货号
enz-51018-0025/ enz-51018-k100
产品名称
mito-id®membrane potential detection kit
规格
1*25tests/1*100tests
短期保存
-20°c
长期保存
-80°c
试剂盒组分
mito-id®mp detection reagent
necrosis detection reagent
cccp control
10x assay buffer 1
50x assay buffer 2
应用
flow cytometry, fluorescence microscopy, fluorescent detection, hts
部分产品引用文献
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