anti-hcn2/fitc抗体使用说明书
form：abcam
catalog number : byk-9858r-fitc
quantity size : 100ug (1mg/ml,lyophilized or liquid.buffer=0.01m pbs,ph 7.4 with 10 mg/ml bsa and 0.1% sodium azide.reconstitute with 0.1ml sterile distilled water.)
function: hyperpolarization-activated, cyclic nucleotide-binding channels (hcn) are voltage-gated cation channels that are activated by direct binding of intracellular cyclic nucleotides. the hcn family consists of four members (hcn1–4), each with a core transmembrane segment domain and a carboxy-terminal 120 amino-acid cyclic nucleotide-binding domain motif (1). hcn channels are expressed in the brain, heart, thalamus and testis (1). the pacemaker properties of hcn channels contribute to spontaneous rhythmic activity in the brain and heart (1). the genes encoding human hcn1 and hcn2 map to chromosomes 5 and 19p13.3, respectively (2,3). the genes encoding hcn3 and hcn4 map to chromosomes 1q21.3 and 15q24-q25, respectively (4,5).
specificity :
anti- hcn2/fitc is a rabbit polyclonal antibody unconjugated specific for hcn2/fitc of human,mouse,rat,dog,pig,rabbit,sheep,bovine use for western blotting,elisa,immunoprecipitation and immunohistochemistryprotein g affinity chromatography purification, purity :>95%isotype: iggmol wt: 97kda
application :
flow-cyt=1:50-200icc=1:50-200if=1:50-200optimal working dilutions must be determined by the end user.
storage: store at -20℃ for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
important note: this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.